Methods: Four cases of metastatic lymph nodes tissue of esophageal cancer were subjected to quantity COL21A1, RUNX2 and COAS gene expression by RQ-PCR.

• 方法:选择 4例经病理证实为食管鳞状细胞癌伴淋巴结转移病例,应用实时荧光定量PCR(RQ- PCR)技术测量其淋巴结转移灶中COL21A1、RUNX2和COAS基因的扩增倍数。