Methods: Flow cytometer(FCM) were used to defect the immunoflourescent stained vWF in pulmonary artery endothelial cells (PAEC) of pig and aortic endothelial cells(AEC) of rats.

• 方法 :利用免疫组化技术对体外培养的猪肺动脉内皮细胞 (PAEC)和大鼠主动脉内皮细胞 (AEC)中vWF进行免疫荧光标记 ,通过流式细胞仪对vWF的细胞阳性率和荧光强度进行定性、定量分析 ,观察低氧和低温所引起的vWF变化。