Methods: (1)Embryonic cerebral cortical neurons of a rat were cultured in vitro by combined method of typspin digestion and DNase I based on the neurobasal medium.

• 第一部分：大鼠胚脑皮质神经元细胞的原代培养、鉴定和缺氧模型的建立目的：进行大鼠胚脑皮质神经元细胞培养和鉴定，并建立大鼠胚脑皮质神经元细胞培养的缺氧模型。