Methods pBP-wt-PTEN recombinant plasmids were transfected into ZR-75-1 cells by Lipofectamine.

• 方法利用脂质体介导法将重组质粒pBP-wt-PTEN导入人乳腺癌细胞ZR-75-1中,嘌呤霉素筛选稳定表达细胞株并扩增培养;