Methods We useed model of swimming exhausition of rats and adopting immunoassay eazyme magnetic analysis(IEMA) method to measure the content of insulin ,C P, to test content of blood glucose and FFA in colorimetric analysis method.

• 方法 采用大鼠游泳力竭模型，分别用磁性微粒分离的免疫酶联测定法（IEMA）测定血清胰岛素、C肽，用比色法测定FFA、血糖，并观察力竭时间和心率的改变。