Methods We perfused donor rat spleens continuously with WZD and selected the best protocol among them,then we examined lymphopoiesis by mixed lymphocyte culture (MLC) and observed ultrastructure of donor splenocytes.

• 方法 以WZD溶液持续灌注移植脾 ,筛选最佳方案 ,并观察灌注后混合淋巴细胞培养(mixedlymphocyteculture ,MLC)中淋巴细胞的增殖情况及灌注后移植脾细胞的超微结构。