Methods We created the model of primary cultured and injured neurons derived from the cortex of the embryo rats.The cells were stained with Trypan Blue to measure the rates of neuronal death.

• 方法运用体外原代胚胎大鼠皮层神经细胞培养技术建立神经细胞损伤的模型,采用台盼兰法测定细胞死亡率,细胞内LDH漏出率评价细胞损伤程度。