Methods VL and VH genes of the mAb LC 1 were cloned into plasmid pSW1 and then plasmid pLC 1 expressing human mouse chimeric Fab fragment gene of the mAb LC 1 was constructed. The plasmid pLC 1 was transformed into E. coli TG 1 and expressed in it.

 
  • 方法将mAbLC 1的VL 和VH 基因插入质粒 pWS1 中 ,构建成表达mAbLC 1人 鼠嵌合Fab片段基因的质粒pLC 1 ,转化大肠杆菌TG 1并在其中表达。
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