Methods VEGF and bFGF as angiogenic factors, thalidomide asan angiogenesis inhibitor, various properties of vascular endothelialcells (RF/6A and SVEC) were detected in vitro with MTT assay,wound migration assay, invasion assay and tube formation assay.

• 方法以VEGF,bFGF为血管新生因子,以Thalidomide血管新生抑制因子对体外培养的血管内皮细胞系(RF/6A,SVEC)应用MTT试验,迁移试验,浸润试验和管状形成试验检测内皮细胞的各种特性;