Methods Using the method for detecting the PMN adhesion to the PMEC,we examined the effects of anti-L-selectin mAb,sulfatide,a ligand of L-selectin on the PMN adhesion to the LPS activated PMEC.

• 方法用组织块培养法培养肺微血管内皮细胞(PMEC),用淋巴细胞分离液分离大鼠PMN,测定用L-选择素单抗、L-选择素配体硫苷脂孵育后的大鼠PMN与不同浓度LPS激活的PMEC间黏附率。