Methods Using carrier c onstruction , plasmid transfection and identificaion,the PEMT-2 overexpression cells was obtained, and protein content and proliferation of cell were analysed by ce ll culture and counts and Coomassie brilliant blue assay.

• 方法 采用载体构建、质粒转染和鉴定方法 ,得到PEMT 2过表达细胞 ,用细胞培养、计数及考马斯亮蓝法分析大鼠肝癌细胞与PEMT 2过表达细胞蛋白含量及增殖情况。