Methods Use glycerine as protective fluid,keep RBC in a low temperature,unfreeze it with water before using,and remove the glycerine with several different washing solutions in concentration.

• 方法以甘油为保护剂,低温保存红细胞,使用前水浴解冻,用不同浓度的洗涤液洗涤去除甘油,以红细胞回收率、游离血红蛋白含量、残余甘油量、体外溶血试验和无菌试验为质量控制项目。