Methods Tyrosinase activity was estimated by measuring the oxidation rate of DL dopa. The inhibitory pattern of each monomers was determined according to their Lineweaver Burk curve as compared to controls.
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方法酪氨酸酶多巴速率氧化法体外测定药物干预前后酪氨酸酶活性,求出酪氨酸酶抑制率,并分别作出相应Lineweaver-Burk曲线,推断其抑制类型。
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