Methods To assess the mRNA expression level of CD59 by RT-PCR and Real-Time PCR in gastric cancer cell lines, gastric cancer tissues and paired nomal-appearing tissues.

• 方法以胃癌细胞系,胃癌组织和癌旁正常配对组织为材料,采用RT-PCR法和Real-Time PCR法测定CD59基因的mR-NA表达水平。