Methods The tlh gene amplified by PCR was cloned into the vector pET32a+ and sequenced, followed by analysis of the biological information by with presenting the sequences to the websites of bioinformatics on the Internet.

• 方法利用PCR技术扩增tlh基因,克隆至载体pET32a+并测序。 将测序结果提交国际互联网上有关生物信息网站进行生物信息学分析。