Methods The synthesized modified antisense ODNs were added to the pancreas cancer cell lines Aspc-1 and Bxpc-3 respectively by gene pluser electroprotocal, telomerase activity was tested by RT-PCR-ELISA, and the cell number were compared by MTT.

• 方法 人工合成端粒酶反义寡核苷酸片段,电转移法将反义端粒酶序列导入胰腺癌细胞株Aspc-1、Bxpc-3中,RT-PCR-ELISA法检测端粒酶活性,MTT法检测细胞活性。