Methods The rat models of hepatic fibrosis were established by human serum albumin(HSA) injection.Serum TNF was detected by RIA method and laminin in the liver was studied by immunohistochemistry.

• 方法采用以人血白蛋白攻击方法制备肝纤维化动物模型,放免法测定血TNF,免疫组化法观察肝内层粘蛋白改变。