Methods The primary VSMCs were cultured by planting Wistar rat thoracic vascular smooth muscle pieces.The proliferation of VSMCs was induced by high concentration of glucose and insulin.

• 方法：组织贴块法培养原代大鼠胸主动脉平滑肌细胞, 用高糖和高胰岛素诱导细胞增殖。