Methods The mRNA from normal lungs was used as template. By using the techniques of RT PCR, nest PCR and cloning, the pUC 19/SP A 6 A clone was established and sequenced.

• 方法以正常人肺组织mRNA为模板 ,采用逆转录反应 (RT) PCR、巢式 PCR及基因克隆技术 ,获得pUC19/SP A 6A克隆 ,并测序证实。