Methods The laying hens aged 30w were immunized with morphine-bovine serum albumin conjugate, and the IgY induced was detected by indirect ELISA.

• 方法用吗啡一牛血清蛋白结合物为模型抗原对30周龄产蛋鸡进行免疫诱导，用ELISA间接法测定不同时间的抗体滴度。