Methods The inhibitory activities of 5-fluorouracil and N1-alkyl-5-fluorouracil derivatives on the U-251 in vitro were observed by trypanblau dyeing under microscope.

• 方法 利用台盼蓝染色显微镜法观察计数方法考查N1-羧酰-5-氟尿嘧啶系列前体药物体外对人星形胶质瘤细胞增殖活性抑制作用；