Methods The human choroidal tissues were digested in two steps by trypsin and collagenase, and the HCEC were obtained and cultured after the digested cell suspension was sorted and purified with magnetic beads of CD31 Dynabeads.

• 方法采用胰酶、胶原酶对人眼脉络膜组织块进行两步消化,应用免疫磁珠CD31 Dynabeads对消化后的细胞混合悬液分选纯化后获得人眼脉络膜血管内皮细胞并进行细胞培养;