Methods The hepatocellular carcinoma cell lines HCC 9903, EGHC 9901,HCC 9901 and HCC 9810 were respectively detected by fluorescence in situ hybridization (FISH) centromere DNA probes of X chromosome.

• 方法 制备三株新建人肝细胞肝癌细胞系 HCC- 990 3、EGHC- 990 1、HCC- 9810染色体标本 ,用 X染色体着丝粒特异 DNA探针 ,对其 X染色体进行荧光原位杂交 (FISH) ,检测 X染色体拷贝数畸变。