Methods The expression of Survivin protein and gene was detected by SP immunohistochemical technique and RT-PCR in 48 cases of cholangiocarcinoma,30 cases of adjacent histologically noncancerous bile duct respectively.

• 方法应用免疫组化SP法检测48例胆管癌和其中30例癌旁胆管组织中Survivin蛋白的表达,同时应用RT-PCR技术检测这些组织标本中Survivin mRNA的表达,并与临床病理资料进行相关性分析。