Methods The antibodies to ouabain were prepared by immunizing rabbits.The optimum antisera titer and concentration of coating buffer with chessboard test and the assay were established.

• 方法利用外源性哇巴因免疫家兔制备哇巴因抗血清，用棋盘试验确定抗血清的稀释度及包被抗原的浓度；