Methods The aged diabetic rats model was induced by D-galactose, enrich-diet and Streptozotocin (STZ low dose) to measure the concentration of SOD and MDA in serum, and detect mRNA and protein expressions of Bax and Bcl-2 genes by RT-PCR and Western-Blot.

• 方法采用D-半乳糖致衰,继而加高脂高糖及注射低剂量链脲佐菌素(STZ)制备衰老DM模型,测定血清中SOD、MDA浓度,并利用RT-PCR法和Western-Blot法检测大鼠胰腺组织凋亡相关基因Bax、Bcl-2mRNA及蛋白表达水平。