Methods The SD rats islets were isolated by digestive method with collagenase and DNase. Millipore Multiscreen Assay System was used to observe the effects of phentolamine on the function of isolated islets.

• 方法 胶原酶和DNA酶联合消化法分离SD大鼠胰岛 ,RP MI164 0组织培养液过夜培养 ,采用MilliporeMultiscreen系统观察不同浓度葡萄糖及酚妥拉明对胰岛功能的影响。