Methods Telomerase activity was detected by a non-isotopic PCR-based TRAP (telomeric repeat amplication protocol) assay in tissue samples from 46 PGC,22 tumor-adjacent tissues and 19 benign lesions.

• 方法采用端粒重复序列扩增法(TRAP)检测了胆囊癌细胞系,46例原发性胆囊癌组织,22例癌旁组织,19例胆囊良性病变。