Methods Survivin-targeting shRNA eukaryotic expression vector pRNAT-U6. 1/neo-survivin was constructed and identified. Human bladder cancer cell T24 was transfect-ed with pRNAT-U6. 1/neo-survivin and then stable monoclonal cells were selected.

• 方法构建Survivin基因shRNA真核表达载体pRNAT-U6.；1/neo-survivin；转染人膀胱癌细胞T24并获得稳定的单克隆细胞。