Methods Single cell suspensions generating from a specimen of primary ependymocytoma were cultured separately in serum-free and serum-supplemented medium to harvest BTSCs and BTCs.

• 方法:取初发的室管膜瘤手术切除标本1例,取材后制备为单细胞,分别接种于无血清培养基获得肿瘤干细胞,接种于含血清培养基获得肿瘤细胞。