Methods Reverse transcriptase polymerase chain reaction(RT-PCR) was applied to detect RV in serum from 200 infants and young children with acute diarrhea(RV positive in stool confirmed by ELISA ).

• 方法大便ELISA一步法证实为急性RV腹泻的不同年龄患儿200例,RT-PCR法检测其血清中RV。