Methods Mouse trachea and pig AEC were cultured in vitro.After CSE exposure, the expression of ECD in AECs was evaluated using immunohistochemical stain and image analysis.

• 方法 体外培养小鼠气管环及猪AEC，加入一定浓度的CSE，应用免疫组化染色和图像分析法检测AEC中ECD表达的变化。