Methods Mouse IL-3 cDNA was cloned to retrovirus vector pLXSN and then the recombinant IL-3 cDNA was transfected on PA317 cells with lipofectin. The cells was selected with C418 after BMS was transfected by the supernatant containing virus.

• 方法 将小鼠IL－3cDNA克隆到逆转录病毒载体（pLX－SN）上，然后将重组的IL－3 cDNA用脂质体转染到包装细胞PA317上，G418筛选后得到抗性克隆，将含病毒的上清液转染骨髓基质细胞。