Methods Molecular biology technique was used to extract mice oocytes, single-cell nested polymerase chain reaction(PCR) was used to detect HBV DNA in oocytes after transfection with PBR322-HBV DNA.

• 方法:用分子生物学技术提取、分离小鼠卵细胞,进行质粒PBR322-HBV DNA的脂质体转染,采用单细胞巢式PCR检测卵细胞中携带的HBV DNA;