Methods Mice were immunized with bovine submaxillary mucin (BSM), the splenic lymphocytes from the immunized mouse were fused with the mouse myeloma Sp2/0 cells using PEG.

• 方法 以牛颌下腺粘蛋白(BSM)为免疫原免疫小鼠,以免疫脾细胞与Sp2/0细胞融合建立杂交瘤细胞株,用有限稀释法克隆化,间接ELISA及间接ELISA结合神经氨酸酶消化筛选克隆。