Methods Mice implanted with H22 tumor cells were injected with 10% carmine, and then vascular index was determined by colorimetry of carmine to evaluate the formation of new blood vessels.

• 方法荷瘤小鼠静脉注射质量分数为10%25的胭脂红溶液，比色法测定肿瘤组织胭脂红含量，计算血管指数反映新生血管形成；