Methods MSK?2 and MS mediums were used to induce gametophytes into callus tissues, and the fuzzy comprehensive evaluation method was used to assess the optimal phytohormone combination.

• 方法用MSK?2和MS培养基对地钱配子体进行愈伤组织诱导，运用模糊评判对MSK?2培养基生长激素组合进行综合性状评价。