Methods In vitro transfered and cultured renal tubular cells AT2 receptor agonist CGP - 42112A in different doses were diffused, then cell apoptosis was analyzed by TUNEL and flow cytometry.

• 方法 通过取新生大鼠肾小管上皮细胞体外培养,传代后加入不同浓度AT_2受体兴奋剂CGP-42112A,在不同时间用流式细胞仪测定凋亡细胞比值,并结合TUNEL分析。