Methods Hep3B cells which lack the expression of both p53 and retinoblastoma tumor suppressor genes because of deletions, were transfected with a wild-type (wt) p53 cDNA and control vector by a liposome method.

• 方法通过采用脂质体介导转染技术，将野生型p53 cDNA导入一种p53和Rb基因缺失的肝癌细胞株Hep3B。