Methods GPSCH, CFA and PT-immunized Wistar rats are divided randomly into four groups, group EAE, group TRD 1, group TRD 2 and group TRD3. The brains and spinal cords were removed for HE staining and in situ hybridizations (ISH) staining.

• 方法将Wistar大鼠免疫后7d随机分成4组:EAE组、TRD1组、TRD2组、TRD3组,给予不同剂量的药物注射,取脑和脊髓制成石蜡切片,进行HE和MCP-1mRNA的原位杂交染色,比较各项临床指标。