Methods Full length of S1 and Prn genes of three vaccine strains were amplified by PCR. The PCR amplicons were sequenced after being cloned into PGEM-T easy vector and the results were compared with those of Bordetella pertussis strains in GenBank.

• 方法分别克隆疫苗株的S1和Prn基因至PGEM-Teasy载体、进行测序,并与GenBank中收录的百日咳杆菌S1和Prn蛋白基因序列进行比较、分析。