Methods Free streptavidin was used to attach the biotinylated second antibody to the Bio-AdAT DNA,through amplication of the DNA lable, Toxoplasma circulating antigens was detected, thus Immuno-PCR assay was established;

• 方法用游离的亲合素将生物素化的二抗和生物素化的腺病毒六邻体基因重组质粒DNA偶联起来,通过PCR扩增标记的DNA检测弓形虫循环抗原,建立了免疫-PCR检测方法;