Methods FQ-nRT-PCR was used to detect BDV p24 gene from CSF and PBMCs of viral encephalitis patients and normal contral of NingXia province.

• 方法用荧光定量套式逆转录酶聚合酶链反应(FQ-nRT-PCR)检测病毒性脑膜脑炎患者及正常人PBMCs和CSF有核细胞BDV p24基因片段,对BDV p24阳性产物FQ-nRT-PCR进行分子克隆和测序分析。