Methods Dissociated neurons were primarily cultured in vitro and the effects of bFGF and EGF on hippocampal neurons were observed in cytomorphology and by MTT method.

• 方法 取新生大鼠海马组织进行原代体外分离培养 ,从细胞形态学方面及应用MTT法观察bFGF和EGF对大鼠海马神经元的影响。