Methods Different concentrations of ATRA were added into culture media system to induce apoptosis of human osteosarcoma cells. The apoptosis was detected by MTT assay,flow cytometry and TUNEL method.

• 方法在培养液体系中加入不同浓度的全反式维甲酸(ATRA)进行不同时间长度的诱导,以MTT法,流式细胞术,TUNEL法检测细胞凋亡情况。