Methods Cytopathogenic effect (CPE) caused by WNV was observed in Vero-E6 and C6/36 cells, and the negative stain and tissue slice samples for electron microscopy were prepared.

• 方法 选择Vero E6与C6 36细胞观察WNV的细胞病变效应 (CPE) ,并制作电镜负染标本和组织切片标本进行病毒形态学鉴定 ;