Methods Culture of clinical isolate of M.chelonei subs p. abscessus inoculated on mycobacteria media slant for 7 days was transferred to a sterile flask containing with glass beads,shake for 5 to 10 minutes,dilute to 108cfu/ml.

• 方法 细菌在分枝杆菌干燥培养基斜面上培养 7d ,转至装有玻珠的小三角瓶中振摇 ,稀释至 10 8cfu/ml。