Methods By RT-PCR,specific amplification products of P2 gene from H2 final product and its parent seed were obtained,then sequenced and blasted to analyze its virulence gene VP1/2A and 2C.

• 方法通过逆转录-聚合酶链反应,特异扩增甲肝减毒活疫苗H2株成品疫苗及其亲本毒种P2区VP1/2A、2B、2C基因序列,经测序、比对,分析检测毒力基因VP1/2A、2C的变异情况。