Methods At first the assay on form of the spores was accomplished, then reducing incubation was used to observe the change of the medium color.

• 方法先进行胞子形成试验,再进行还原性培养观察颜色变化,培养基颜色变黑则表示亚硫酸钠被还原,可判定厌氧芽胞菌阳性。