Methods An open reading frame of human BSP was amplified by PCR method and was reconstructed into the eukaryotic expressive vector pIRES2-EGFP to construct the hBSP sense or antisense expressive vector .

• 方法以PCR的方法扩增人BSP的开放阅读框序列,与载体pIRES2-EGFP相连构成重组正反义表达质粒。